RESEARCH ARTICLE


Injection of Human Bone Marrow and Mononuclear Cell Extract into Infarcted Mouse Hearts Results in Functional Improvement



Franca S Angeli 1, #, Yan Zhang 1, #, Richard Sievers 1, Kristine Jun 1, Sarah Yim 1, Andrew Boyle 1, 2, Yerem Yeghiazarians 1, 2, *
1 Division of Cardiology, Department of Medicine, University of California, San Francisco, USA
2 Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, USA


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© Angeli et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Division of Cardiology, Department of Medicine; Box 0103, Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, CA 94143-0103, USA; Tel: 415-353-3817; Fax: 415-353-3090; E-mail: yeghiaza@medicine.ucsf.edu
# Both authors contributed equally to this work.


Abstract

Background:

We have previously shown that mouse whole bone marrow cell (BMC) extract results in improvement of cardiac function and decreases scar size in a mouse model of myocardial infarction (MI), in the absence of intact cells. It is not clear if these results are translatable to extracts from human BMC (hBMC) or mononuclear cells (hMNC), which would have significant clinical implications.

Methods:

Male C57BL/6J (10-12 weeks old) mice were included in this study. MI was created by permanent ligation of the left anterior descending artery. Animals were randomized into three groups to receive ultrasound-guided myocardial injections with either hBMCs extract (n=6), hMNCs extract (n=8) or control with 0.5% bovine serum albumin (BSA) (n=7). Cardiac function was assessed by echocardiography at baseline, 2 and 28 days post-MI. Infarct size and vascularity was assessed at 28 days post-MI.

Results:

hBMC and hMNC extract preserve cardiac function and result in smaller scar size post-MI when compared with the control group.

Conclusions:

The current study for the first time reports that hBMC and hMNC extracts improve cardiac function post-MI in a mouse MI model. Further studies are necessary to fully address the potential clinical benefits of these therapies.

Keywords: Bone marrow cells, stem cell therapy, paracrine effect, myocardial infarct..