Oxidative Stress As A Common Mediator for Apoptosis Induced-Cardiac Damage in Diabetic Rats

Mohammad M Dallak1, Dimitri P Mikhailidis2, *, Mohamed A Haidara1, Ismaeel M Bin-Jaliah1, Olaa M Tork3, Moshira A Rateb3, Hanaa Z Yassin3, Zeinb A Al-refaie3, Ibrahim M Ibrahim3, Samy M Elawa4, Laila A Rashed5, Noha A Afifi6
1 Physiology Department, College of Medicine, King Khalid University, Saudi Arabia
2 Department of Clinical Biochemistry (Vascular Prevention Clinic), Royal Free Hospital campus, University College of London, UK
3 Physiology Department, Faculty of Medicine, Cairo University, Egypt
4 College of Health and Sciences, Kuwait
5 BiochemistryDepartment, Faculty of Medicine, Cairo University, Egypt
6 Histology Department, Faculty of Medicine, CairoUniversity, Egypt

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© Dallak et al.; Licensee Bentham Open

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution License (,which permits unrestrictive use, distribution, and reproduction in any medium, provided the original work is properly cited

* Address correspondence to this author at the Department of Clinical Biochemistry (Vascular Prevention Clinic), Royal Free Hospital campus, University College of London, UK; Tel: 0044(0) 20 78302258; E-mail:



To investigate the possible role of oxidative stress as a common mediator of apoptosis and cardiac damage in diabetes.

Materials and Methods:

This experimental work was conducted on 5 groups of Wistar rats. Group I was the control group. Diabetes type 1 was induced in other groups (by streptozotocin) and animals received insulin or vitamin E (300 mg /kg body weight), both insulin and vitamin E, or no treatment for 4 weeks according to their group. At the end of the study, serum and cardiac tissues were examined for biochemical parameters of cardiac function, oxidative stress and apoptosis. Electron microscopy pictures of cardiac tissue were also evaluated for signs of cardiac damage


Markers of oxidative stress, apoptosis, inflammation as well as manifestations of cardiac damage as assessed by electron microscopy were significantly decreased in rats treated with both insulin and vitamin E when compared with untreated diabetic rats or rats treated with either insulin or vitamin E alone


Administration of both vitamin E and insulin was effective in reducing markers of oxidative stress and apoptosis and improving parameters of cardiac function in experiments animals. Antioxidants might prove beneficial as an adjuvant treatment in addition to insulin in type 1 diabetes associated with manifestations of cardiac complications

Key Words: Diabetes, vitamin E, Wistar rats, diabetic cardiomyopathy, apoptosis, oxidative stress, cardiac enzymes.