RESEARCH ARTICLE


Oxidative DNA Damage in Blood of CVD Patients Taking Detralex



Krzyściak Wirginiaa, b, *, Cierniak Agnieszkac, Kózka Mariuszd, Kozieł Joannae
a Department of Medical Diagnostics, Jagiellonian University, Medical College, Pharmacy Faculty, Krakow, Poland
b Department of Radioligand, Chair of Pharmacobiology, Jagiellonian University, Medical College, Pharmacy Faculty, Krakow, Poland
c Department of General Biochemistry; Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 7 Gronostajowa Str., 30-387 Krakow, Poland
d Department of General Surgery, Jagiellonian University Medical College, 21 Kopernika Str., 31-501 Krakow, Poland
e Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 7 Gronostajowa Str., 30-387 Krakow, Poland

Article Information


Identifiers and Pagination:

Year: 2011
Volume: 5
First Page: 179
Last Page: 187
Publisher ID: TOCMJ-5-179
DOI: 10.2174/1874192401105010179

Article History:

Received Date: 18/4/2011
Revision Received Date: 29/4/2011
Acceptance Date: 30/4/2011
Electronic publication date: 01/8/2011
Collection year: 2011

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Creative Commons License
© Wirginia et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Department of Medical Diagnostics, Pharmacy Faculty, UJCM, 9 Medyczna Str. 30-688 Krakow, Poland; Tel: +4812 6205760; Fax: +4812 6205400; E-mail: wirginiakrzysciak@cm-uj.krakow.pl


Abstract

The main goal of the work reported here was to determine the degree of oxidative/alkali-labile DNA damages in peripheral blood as well as in the blood stasis from varicose vein of (chronic venous disorder) CVD patients. Moreover, determination of the impact of Detralex usage on the level of (oxidative) DNA damages in CVD patients was evaluated as well.

The degree of oxidative DNA damages was studied in a group consisted of thirty patients with diagnosed chronic venous insufficiency (CVI) in the 2nd and 3rd degree, according to clinical state, etiology, anatomy and pathophysiology (CEAP), and qualified to surgical procedure. The control group consisted of normal volunteers (blood donors) qualified during standard examinations at Regional Centers of Blood Donation and Blood Therapy.

The comet assay was used for determination of DNA damages.

Analyses of the obtained results showed increase in the level of oxidative/alkali-labile DNA damages in lymphocytes originating from antebrachial blood of CVD patients as compared to the control group (Control) (p < 0.002; ANOVA). In addition, it was demonstrated that the usage of Detralex® resulted in decrease of the level of oxidative/alkali-labile DNA damages in CVD patients as compared to patients without Detralex® treatment (p < 0.001; ANOVA).

Based on findings from the study, it may be hypothesized about occurrence of significant oxidative DNA damages as the consequence of strong oxidative stress in CVD. In addition, antioxidative effectiveness of Detralexu® was observed at the recommended dose, one tablet twice daily.

Keywords: Reactive oxygen species (ROS), chronic venous insufficiency (CVI), micronized purified flavonoid fraction (MPFF)..